An aliquot of the extract was mixed with 5ml Folin-Ciocalteu reagent (previously diluted with water at 1:10 v/v) and 4ml (75 g/l) of sodium carbonate.
The tubes were vortexed for 15 sec and allowed to stand for 30 min at 40 degree Celsius for color development.
Absorbency was then measured at 765nm using UV-VS spectrophotometer.
Samples evaluated at a final concentration of 0.1mg/ml.
Total phenolic content were expressed as mg/g Gallic acid equivalents using the following equation based on the calibration curve: y = 0.1216x, R2 = 0.9365,
The tubes were vortexed for 15 sec and allowed to stand for 30 min at 40 degree Celsius for color development.
Absorbency was then measured at 765nm using UV-VS spectrophotometer.
Samples evaluated at a final concentration of 0.1mg/ml.
Total phenolic content were expressed as mg/g Gallic acid equivalents using the following equation based on the calibration curve: y = 0.1216x, R2 = 0.9365,
o Where x was the absorbance and y was the Gallic acid equivalent (mg/g).
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