Antioxidant Activity by DPPH radical scavenging assay

The effect of the Antioxidant potential of samples by using DPPH radical scavenging activity  by using the method according to Liyana-Pathiranan and Shahidi [29].

1. A solution of 0.135mM DPPH in methanol was prepared and 1.0 ml of this solution was mixed with 1.0 ml of extract in methanol containing 1 mg of the sample.
2. The reaction mixture left in the dark at room temperature for 30min.
3. The absorbance of the mixture was measured spectrophotometrically at 517nm.
4. BHT was used as reference.
5. The ability to scavenge DPPH radical was calculated by the following equation:
6. DPPH radical scavenging activity (%) = [(Abscontrol - Abssample)]/ (Abscontrol)] x 100

o    where Abscontrol is the absorbance of DPPH radical + methanol;

o    Abssample is the absorbance of DPPH radical + sample extract /standard.